Description
The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX100 or QX200 Droplet Reader.
The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time: it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
Features- Guides setup and loading with large, color touch-screen interface and LED lighting
- Generates droplets for 96 ddPCR reactions in under 45 minutes
- One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
- Provides operational flexibility:
- User-definable plate layout with increments of 8 wells
- HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
- Compatible with both QX100 and QX200 ddPCR workflows
Applications
- Cancer biomarker studies and copy number variation
- Pathogen detection
- Next generation sequencing
- Gene expression analysis
- Environmental monitoring
- Food testing